The role of gene Nu3 in bacteriophage lambda head morphogenesis
Identifieur interne : 005376 ( Main/Exploration ); précédent : 005375; suivant : 005377The role of gene Nu3 in bacteriophage lambda head morphogenesis
Auteurs : Peter Ray [Canada] ; Helios Murialdo [Chili]Source :
- Virology [ 0042-6822 ] ; 1975.
Abstract
The role of gene Nu3 in λ head morphogenesis has been investigated using a recently isolated amber mutant (ama8) in this gene. Analysis of lysates of sup− cells infected with λama8 using electron microscopy, revealed the presence of a relatively high proportion of “monsters.” In this respect, such lysates resemble those obtained from λBamCam infected cells. The nature of the gene Nu3 product, and its involvement in several interactions with other morphogenetic proteins, were examined by SDS-polyacrylamide gel electrophoresis, followed by autoradiography of radioactively labeled cell extracts. The product of gene Nu3, pNu33 3 Abbreviations used: pA, pB, pC, etc. represent the primary protein products of genes A, B, C, etc.; SDS, sodium dodecylsulfate; E/M, electron microscopic., is a polypeptide of 19,000 molecular weight. It appears to be bound to precursor head structures very early during assembly, but is subsequently released and cleaved. pNu3 is required for the functional assembly and cleavage of the products of genes B and C. In Nu3 mutants neither pB nor pC are found associated with the nascent head structures, known as petit λ. On the basis of these data and of results published by others a model is proposed to account for the interactions that take place among morphogenetic gene-products during the early events in head morphogenesis.
Url:
DOI: 10.1016/0042-6822(75)90096-3
Affiliations:
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Analysis of lysates of sup− cells infected with λama8 using electron microscopy, revealed the presence of a relatively high proportion of “monsters.” In this respect, such lysates resemble those obtained from λBamCam infected cells. The nature of the gene Nu3 product, and its involvement in several interactions with other morphogenetic proteins, were examined by SDS-polyacrylamide gel electrophoresis, followed by autoradiography of radioactively labeled cell extracts. The product of gene Nu3, pNu33 3 Abbreviations used: pA, pB, pC, etc. represent the primary protein products of genes A, B, C, etc.; SDS, sodium dodecylsulfate; E/M, electron microscopic., is a polypeptide of 19,000 molecular weight. It appears to be bound to precursor head structures very early during assembly, but is subsequently released and cleaved. pNu3 is required for the functional assembly and cleavage of the products of genes B and C. In Nu3 mutants neither pB nor pC are found associated with the nascent head structures, known as petit λ. On the basis of these data and of results published by others a model is proposed to account for the interactions that take place among morphogenetic gene-products during the early events in head morphogenesis.</div></front></TEI><affiliations><list><country><li>Canada</li><li>Chili</li></country><region><li>Ontario</li></region><settlement><li>Toronto</li></settlement><orgName><li>Université de Toronto</li></orgName></list><tree><country name="Canada"><region name="Ontario"><name sortKey="Ray, Peter" sort="Ray, Peter" uniqKey="Ray P" first="Peter" last="Ray">Peter Ray</name></region></country><country name="Chili"><noRegion><name sortKey="Murialdo, Helios" sort="Murialdo, Helios" uniqKey="Murialdo H" first="Helios" last="Murialdo">Helios Murialdo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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